Chen jing ke license#

1-NP caused a significantly concentration-dependent and time-dependent viability decrease. The combined toxic effects of 1-NP and 1,2-naphthoquinone (1,2-NQ) on A549 were also evaluated. Using human lung epithelial A549 cells, viability was measured by MTT assay after treated with 1-nitropyrene (1-NP) lactate dehydrogenase (LDH) leakage was determined to evaluate the cellular membrane injury DNA damage was detected with comet assay reactive oxygen species (ROS) generation was measured with fluorescent probe.

Furthermore, the phylogenetic analysis of the dioxygenase alpha subunits showed that TcbAa was belong to the toluene/diphenyl subfamily, and was most closely related to the poly-chlorinated benzene dioxygenase alpha subunit.

By alignment of the amino acid sequences of the a subunits of TcbAa (from J5-1) and TecA1 (from PS12), four amino acid residues from site 307 to site 310 were found to be different (I307L, M308T, I309V, Q310E), which probably retarded the preference for the substrate 1,2,4,5-tetrachlorobenzene. Homology analysis indicated that these genes and gene products were most closely related to those of Burkholderia sp. PCR technique was used to amplify the genes of chlorobenzene dioxygenase and dehydrogenase of J5-1, and they were named as tcbA and tcbB, respectively. Pseudomonas nitroreducens J5-1 is able to use monochlorobenzene, 1,2-dichlorobenzene, 1,3-dichlorobenzene and 1,2,4-trichlorobenzene as sole carbon and energy sources, and it differs from those 1,2,4-trichlorobenzene degrading bacteria reported in substrate utilizing characters. The results will be of great significance for developing efficient and in-situ remediation technology of DDT contaminated soil. There was no significant interaction between butyrate and iron oxide on DDT dechlorination, whereas there was antagonistic action between ethanol and iron oxide on DDT dechlorination. The applications of only goethite or goethite and electron donor substances resulted in the increased soil pH, decreased soil Eh and increased Fe( II ) contents, thus accelerated DDT dechlorination. During the prophase of incubation, the application of butyrate or ethanol led to the decreased pH and increased Eh for reaction system, thus inhibited DDT dechlorination. The main product of DDT anaerobic dechlorination was 1,1-dichloro-2,2-bis (p-chloro-phenyl) ethane (DDD). Results showed that after 6 weeks of anaerobic incubation, the extractable residues of DDT were between 1.29% and 2.01% of initial DDT amounts in soils, which was attributed to the dechlorinated degradation of DDT and formation of bound residues of DDT and its dechlorinated products. Anaerobic soil incubation experiment was conducted to study the effect and its mechanism of iron oxide (goethite), electron donor substances (butyrate and ethanol), and their interaction on DDT reductive dechlorination in Hydragric Acrisols.

If you do not agree to these terms and conditions, do not download, display or use the image.The interaction between iron oxide and electron donor substance have significant influences on electron transfer and the growth of iron-reducing bacteria, which may affect the reductive dechlorination of polychlorinated organic compounds in soil. Please credit the Lawrence Berkeley National Laboratory and provide the following copyright notice: '© 2010 The Regents of the University of California, through the Lawrence Berkeley National Laboratory.' You agree that the image selected by you may be used for noncommercial, educational purposes only no derivative works are allowed, photos of individuals may only be used for identifying the individual and/or their research, and attribution and copyright notice is required.

Chen jing ke license#

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